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qubit high sensitivity double stranded dna assay  (Thermo Fisher)


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    Structured Review

    Thermo Fisher qubit high sensitivity double stranded dna assay
    Qubit High Sensitivity Double Stranded Dna Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qubit+double+stranded+dna+high+sensitivity+assay/Deoxyribonucleic+acid/pmc12853167-333-9-15
    Average 99 stars, based on 1 article reviews
    qubit high sensitivity double stranded dna assay - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: A custom hybridisation enrichment forensic intelligence panel to infer biogeographic ancestry, hair and eye colour, and Y chromosome lineage.
    Article Snippet: Massively parallel sequencing can provide genetic data for hundreds to thousands of loci in a single assay for various types of forensic testing.. However, available commercial kits require an initial PCR amplification of short-to-medium sized targets which limits their application for highly degraded DNA.. Development and optimisation of large PCR multiplexes also prevents creation of custom panels that target different suites of markers for identity, biogeographic ancestry, phenotype, and lineage markers (Y-chromosome and mtDNA).

    Agarose Gel Electrophoresis:

    Article Title: A custom hybridisation enrichment forensic intelligence panel to infer biogeographic ancestry, hair and eye colour, and Y chromosome lineage.
    Article Snippet: Massively parallel sequencing can provide genetic data for hundreds to thousands of loci in a single assay for various types of forensic testing.. However, available commercial kits require an initial PCR amplification of short-to-medium sized targets which limits their application for highly degraded DNA.. Development and optimisation of large PCR multiplexes also prevents creation of custom panels that target different suites of markers for identity, biogeographic ancestry, phenotype, and lineage markers (Y-chromosome and mtDNA).

    Sensitive Assay:

    Article Title: A custom hybridisation enrichment forensic intelligence panel to infer biogeographic ancestry, hair and eye colour, and Y chromosome lineage.
    Article Snippet: Massively parallel sequencing can provide genetic data for hundreds to thousands of loci in a single assay for various types of forensic testing.. However, available commercial kits require an initial PCR amplification of short-to-medium sized targets which limits their application for highly degraded DNA.. Development and optimisation of large PCR multiplexes also prevents creation of custom panels that target different suites of markers for identity, biogeographic ancestry, phenotype, and lineage markers (Y-chromosome and mtDNA).

    Article Title: Genomic profiling of idiopathic peri-hilar cholangiocarcinoma reveals new targets and mutational pathways
    Article Snippet: DNA was extracted using DNEasy (Qiagen, Venlo, Netherlands). .. FFPE-derived DNA samples were quantified by Qubit double stranded DNA high sensitivity assay (Thermofisher Scientific, Waltham, Massachusetts, USA). .. An Agilent Next Generation Sequencing FFPE QC kit (Agilent Technologies, Santa Clara, California, USA) was used to quantify and qualify the DNA by qPCR with exome sequencing on Illumina HiSeq 4000 (version 1 chemistry) (Illumina, San Diego, California, USA), generating 2 × 150 base pair paired end read libraries.

    other:

    Article Title: Long noncoding RNA LEENE promotes angiogenesis and ischemic recovery in diabetes models
    Article Snippet: Subsequently, ChIRP-seq libraries were constructed using the KAPA HyperPrep Kit (Roche Diagnostics) following the manufacturer’s manual.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Genomic profiling of idiopathic peri-hilar cholangiocarcinoma reveals new targets and mutational pathways
    Article Snippet: DNA was extracted using DNEasy (Qiagen, Venlo, Netherlands). .. FFPE-derived DNA samples were quantified by Qubit double stranded DNA high sensitivity assay (Thermofisher Scientific, Waltham, Massachusetts, USA). .. An Agilent Next Generation Sequencing FFPE QC kit (Agilent Technologies, Santa Clara, California, USA) was used to quantify and qualify the DNA by qPCR with exome sequencing on Illumina HiSeq 4000 (version 1 chemistry) (Illumina, San Diego, California, USA), generating 2 × 150 base pair paired end read libraries.

    Nucleic Acid Electrophoresis:

    Article Title: Analytical validation of an error-corrected ultra-sensitive ctDNA next-generation sequencing assay.
    Article Snippet: Plasma and PBMCs were stored at -80◦C until batch processing. cfDNA was extracted using the QIAamp circulating nucleic acid kit (Qiagen, Hilden, Germany) and eluted in 50 μl buffer AVE. Because cfDNA fragments are between 150 and 500 bp in length [1], contaminating large molecular weight fragments (>500 bp) were removed using a 0.5:1 ratio of AMPure XP paramagnetic beads (Beckman Coulter, CA, USA) [11]. .. The size distribution, quality and quantity of the resulting cfDNA were assessed using automated gel electrophoresis (Agilent TapeStation 2200 with High Sensitivity D1000 ScreenTapes, and Expert software; Agilent, CA, USA) and the Qubit double-stranded DNA high-sensitivity assay (Thermo Fisher Scientific, MA, USA). .. Germline DNA was extracted from PBMCs using the QIAamp DNA blood mini kit (Qiagen) and eluted in 200μl buffer AE.

    Software:

    Article Title: Analytical validation of an error-corrected ultra-sensitive ctDNA next-generation sequencing assay.
    Article Snippet: Plasma and PBMCs were stored at -80◦C until batch processing. cfDNA was extracted using the QIAamp circulating nucleic acid kit (Qiagen, Hilden, Germany) and eluted in 50 μl buffer AVE. Because cfDNA fragments are between 150 and 500 bp in length [1], contaminating large molecular weight fragments (>500 bp) were removed using a 0.5:1 ratio of AMPure XP paramagnetic beads (Beckman Coulter, CA, USA) [11]. .. The size distribution, quality and quantity of the resulting cfDNA were assessed using automated gel electrophoresis (Agilent TapeStation 2200 with High Sensitivity D1000 ScreenTapes, and Expert software; Agilent, CA, USA) and the Qubit double-stranded DNA high-sensitivity assay (Thermo Fisher Scientific, MA, USA). .. Germline DNA was extracted from PBMCs using the QIAamp DNA blood mini kit (Qiagen) and eluted in 200μl buffer AE.



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